Genealogical Experiment of Fish Types

Genealogical Experiment of Fish Types David Hess Abstract: To introduce us to proteins, which truly make one organism different from another in terms of phenotype, our instructor challenged us to study the physical and proteomic traits of salmon, catfish, turbot, halibut, and yellow-fin tuna and estimate how each fish is related on the evolutionary tree. To do so, our lab group first accessed online research websites to compare the phenotypes of the different Ichthyoids. We specifically researched sizes, swim types, biological features, habitat preferences, and taxonomic names that derive from the evolutionary tree. After this, we then obtained samples of the muscle tissue in each fish, which were provided by the instructor, and then extracted the proteins from each sample. By treating the samples with sodium dodecyl sulfate and applying heat. We were able to denature the[D1] tertiary and quaternary structures of the proteins, which left the proteins long, stringy, and negatively charged. Next, we were able to separate the proteins by length a la gel electrophoresis, and compare the different proteins in the fish as we observed the different bars that appeared on the gel. After comparing both the physical traits and the proteins in each fish, we were to predict which species preceded the next according to evolution. Purpose: The purpose of this lab is to study the physical attributes and proteomics of different species of fish to determine the potential genealogical tree connecting these species Instructor/Background: Proteins often bind together, forming polypeptide chains. Some atoms on these chains are hydrophilic, while others are hydrophobic. This is due to the fact that the different r-groups (the only part of an amino acids that distinguishes it from another), may or may not form hydrogen bonds with the water molecules that they are summered in. When a hydrophobic group enters the body of water, the hydrogen bonds in the water break apart, yet cannot bind to the r group on the amino acids, so the water forms bonds with itself again around the r-group, thus pushing the r-group away due to the magnetic forces that push similarly charged atoms away from eachother. However, if a hydrophilic group is exposed to water, hydrogen bonds are formed with the r-group, pulling the r-group out of the remaining protein structure due to magnetic forces pulling the two bodies together as they are oppositely charged. These two interactions cause the protein to bundle up, making it hard to perform accurate ge l electrophoresis on. It becomes especially difficult when these proteins bind together with disulfide bonds. Heat and sodium dodecyl sulfate break apart the disulfide and hydrogen bonds. This allows us to separate the proteins in electrophoresis, which can then be compared. [A] Data/Organization [D2]of Records: The following data results from reseach using the Fishbase website to compare phenotypes between the studied fish Common Name: Salmon Scientific Fish: Oncorhynchus Keta Taxonomic Classification: Family Salmonidae (Salmonids) Order Salmoniforms (Salmons) Class Actinopterygii (Ray-Finned Fish) Size: Max Published Weight: 15.9kg Environment: Marine; Freshwater; Brackish; Benthopelagic; Anadromous Depth Range: 0-250m Biology: Inhabits Ocean and Coastal streams. Adults cease eating in freshwater. Die After Spawning. Migrating fry forms schools in estuaries, remain close to shore for a few months, and disperse and enter into the sea. Epilegic. Swim Type: Anguilliform (Moves Body and Caudal Fin) Additional Factors: Definitions of Unfamiliar Terms: Epilogic-Living in the upper zone of the ocean from just below the surface to about 100m in depth Common Name: Halibut Scientific Fish: Hippoglossus Hippoglossus Taxonomic Classification: Family Pleuronectidae (Right-Eye Flounders) Order Pleuronectiformes (Flatfish) Class Actinopterygii (Ray-finned Fish) Size: Max Recorded Length: 470.0cm Max Recorded Weight: 320.0kg Environment: Marine; Demersal Depth: 50-2000m Biology: Adults are Benthic, but occasionally Pelagic. Feeds mainly one other fishes, but also eats cephalopods, large crustaceans, and other bottom-living animals. Seriously affected by overfishing Swim Type: Anguilliform: Body and Caudal Fin Additional Factors: Dorsal Spines Definitions of Unfamiliar Terms: Benthic: Lives one the bottom of a body of water Pelagic: Lives far away from land Common Name: Catfish Scientific Fish: Neoprius Graeffei Taxonomic Classification: Family Arildae (Sea Catfishes) Order Siluriformes (Catfish) Class Actinopterygii (Ray-Finned Fishes) Size: Max Length 60.0cm Environment: Marine; Freshwater; Brackish; Demersal PH Range 7.5-8.2 Anadromous Biology: Inhibit freshwater rivers and lagoons, Brackish estuaries, coastal marine waters. Feeds on arthropods, insects, aquatic plants, mollusks, prawns, crayfish, fishes, and bottom detritus Swim Type: Anguilliform (moves body and caudal fin) Additional Factors: 1 Dorsal spine, 7 dorsal soft rays, and 15-19 soft anal spines Definitions of Unfamiliar Terms: Anadromous: Migrates from freshwater to spawn in salt-water Common Name: Yellowfin Tuna Scientific Fish: Thunaus Albacarares Taxonomic Classification: Family Scombridae (Mackerels, Tunas, Bonitos) Order Perciformes (Perch-Likes) Class Actinopterygii (Ray-Finned Fishes) Size: Max Weight: 200.0kg Max Length: 230.0cm Environment: Marine; Brackish; Pelagic-Oceanic; Oceandromous Depth Range: 1-250m Biology: Lives above and below thermoclines, Pelagic in open water, rarely seen around reefs, school by size, large fish school with porpoise, sensitive to low concentrations of oxygen, resides near ocean debris Swim Type: Anguilliform (Movements of body and/or Caudal fin) Additional Factors: 11-14 Dorsal Rays, 12-16 Dorsal soft rays,11-16 Anal Soft Rays, 39 Vertebrae Definitions of Unfamiliar Terms: Common Name: Turbot Scientific Fish: Scophthalmus Maximus Taxonomic Classification: Family Actinopterygii (Ray-Finned Fish) Order Pleuronectiformes (Flatfish) Class Actinopterygii (Ray-Finned Fish) Size: Max Published Weight: 25.0kg Environment: Marine; Brackish; Demersal; Oceandromous; Temperate Depth Range: 20-70m Biology: Live one sand, rock, or mixed bottom. Almost Circular Bottom. Eye side without scales, but instead bony tubercles. Feeds one bottom-living fishes (sand eels, gobies, etc.) and larger crustaceans and bivalves. Lives especially in Brackish Waters Swim Type: Anguilliform: Movements of body and/or caudal fin Additional Factors: Larvae are initially systematic, but after 40-50 days, the right eye moves to its left side. Definitions of Unfamiliar Terms: Oceandromous: migratory one salt-water Upon the conclusion of our lab, we obtained a gel with protein bands that looked like this: The following graph shows a standard curve based on the distance that the bars travelled and the weight of said bars: The following table describes the distances various bands of proteins moved down their wells. We would use this information to calculate the weight of these bands by comparing them to our standard curve:[D3] By using the band distances and the standard curves that we made, we were able to calculate the weight of these protein bands in Kilo Daltons: By comparing the bands on the gel, our lab group made the following tables showing which fish had certain proteins in their muscles tissue. *Each, â€Å"X† represents the presence of the mentioned protein on the left-hand side of the table in the fish This table compares the proteins located in the chart above, and shows the similarities of proteins between the species. Results: Upon the completion of the analysis of our results, we obtained the following Celptogram[D4]. We knew that Species E only shared a common protein with species B, so it needed to be on one of the ends of the Cleptogram[D5]. We also noted that species C and D shared multiple common proteins in common, so they needed to be close together on the tree. During our analysis of the proteins, our teacher identified which letter represented each fish (it had remained a blind experiment up till this point) as the following: Fish A-Salmon Fish B-Yellow fin Tuna Fish C-Halibut Fish D-Turbot Fish E-Catfish With this extra information, we were able to analyze both our results and the evolutionary tree to create the cleptogram. For example, we noticed that species C and D both had a similarity with D, so we looked at the evolutionary tree to measure whether Tuna or Halibut were closer to Salmon evolutionarily to finish our prediction. Discussion: When reviewing the data once more, we noticed some discrepancies in our cladogram compared to the evolutionary tree. For example, our Yellow-fin Tuna found its way onto the beginning of the tree, when it should have landed near the end according to the evolution tree in our packet. Otherwise, we believe this experiment [D6]was a success, as we learned about how proteins can be used to supplement genetics and give us another tool in understanding our history. This could possibly be result of contamination in the fish muscle samples, due to touching the muscles with the same pair of gloves when transferring them into the tunes for protein extraction. If we were ever to do this experiment again, we would be sure to use tweezers of another similar tool to handle the muscles. Work cited â€Å"Hydrophobic_Interactions†http://chemwiki.ucdavis.edu/Physical_Chemistry/Physical_Properties_of_Matter/Atomic_and_Molecular_Properties/Intermolecular_Forces/Hydrophobic_interactions [D1]Include secondary here as well. Get this published! http://www.journys.org/content/procedures [D2]â€Å"Could this have been organized into a data table which contains all the fish and is still able to describe these different features of the bioinformatics? Thank you for getting the bioinformatics in here! [D3]Good connection between data sets. [D4]cladeogram [D5]? [D6]Great work! This experiment went swimmingly! Hah!

Legislation Legacy

Native Americans or the so called American Indians are considered to belong in the minority group by the White Americans.   They were in one way said to be like a third world country because they have a slow pacing in terms of civilization and improvement. Native Americans are faced with a continuous struggle regarding land ownership. (Komp, 2006)Their lands as they say had a spiritual attachment and they really value the piece of land that they own. Any action that would entail the sale and destruction of the land they own would cause great apprehension. This could not be realized abruptly due to the nature of the Natives to be submissive. They needed to have a strong and powerful person to assist them in kinds of need. The issue between the Native Americans and the federal government is about the acquisition of land. They were at all cost hindered to have a land of their own. The government, despite the legislature that they must acquire land, was trying to bend the rule that’s why the case is in pending condition. This scenario could be accounted to the fact that white Americans have the tendency to take advantage over the natives because they were thinking that the natives do not contribute much to the making of their nation. The issue is connected to the fact that the land of the native Americans are proposed by industries to be the site for toxic wastes disposal.(Komp, 2006)If this would be the situation, then most of the American Indians are now faced with the great struggle regarding their health condition. Although some scientist has cited that it would never affect their health, there is no concrete evidence that this is not harmful to them. The legislation that is linked to the issue is the human rights law.   American Indians, though native, have the right to protect their possessions and to have a land of their own. Also they have the right to be protected in terms of health. And the fact that they were natives must not deprive them of the right to live in a safe environment. References: Komp, K. (2006). Congress, President Still Ignoring the Indian Health Care [Electronic Version]. Retrieved August 6, 2007.      

Analysis Of Brutus In The Tragedy Of Julius Caesar

Trent Shelton once said, â€Å"Sometimes the people you love the most, turn out to be the people you can trust the least.† Trent Shelton expresses in this quote that you can never be too careful when it comes to trust because people change so quickly. A prime example of this is a character named Brutus in William Shakespeares The Tragedy of Julius Caesar. In this play the noble Brutus is persuaded by a group of conspirators that Julius Caesar, a friend of Brutus, should be killed before he is crowned the king. Following the death of Caesar, Brutus speaks out to the people of Rome. He left his speech feeling extremely confident, but soon came to find an angry city upset by the death of their future king. Consequently, Brutus and the other†¦show more content†¦He lets the words of others convince him easily because of his trusting personality. Even though he has the right intentions, he allows his trust in Cassius to get in the way of those intentions. Brutus’ de cision to trust in Cassius and join the conspiracy caused him to lose everything. This included a friend, the trust of Rome, and eventually his life. Brutus also reveals his trusting nature after he learns about Cassius ´ plan to assassinate Caesar. He decides to put aside his love for Caesar and follow through with Cassius’ proposal. â€Å" That at his will he may do danger with with. /Th’ abuse of greatness is when it disjoins / Remorse from power; and, to speak truth of Caesar, /I have not known when his affections swayed /More than his reason. But ‘tis a common proof.† (2. 1. 17-21). Brutus is saying that if Caesar takes the crown, the power will get to him and there is no telling what he may do. The words that Brutus spoke were the words Cassius used to convince Brutus to join the conspiracy for the good of Rome. Brutus is now starting to believe that killing Caesar is the best thing for Rome. This reveals that he trusted Cassius through adopting his ideas. 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How To Write an Essay

Writing an essay is like making a hamburger. Think of the introduction and conclusion as the bun, with the meat of your argument in between. The introduction is where youll state your thesis, while the conclusion sums up your case. Both should be no more than a few sentences. The body of your essay, where youll present facts to support your position, must be much more substantial, usually three paragraphs. Like making a hamburger, writing a good essay takes preparation. Lets get started! Structuring the Essay (aka Building a Burger) Think about a hamburger for a moment. What are its three main components? Theres a bun on top and a bun on the bottom. In the middle, youll find the hamburger itself. So what does that have to do with an essay? Think of it this way: The top bun contains your introduction and topic statement. This paragraph begins with a hook, or factual statement intended to grab the readers attention. It is followed by a thesis statement, an assertion that you intend to prove in the body of the essay that follows.The meat in the middle, called the body of the essay, is where youll offer evidence in support of your topic or thesis. It should be three to five paragraphs in length, with each offering a main idea that is backed up by two or three statements of support.The bottom bun is the conclusion, which sums up the arguments youve made in the body of the essay. Like the two pieces of a hamburger bun, the introduction and conclusion should be similar in tone, brief enough to convey your topic but substantial enough to frame the issue that youll articulate in the meat, or body of the essay. Choosing a Topic Before you can begin writing, youll need to choose a topic for your essay, ideally one that youre already interested in.  Nothing is harder than trying to write about something you dont care about. Your topic should be broad or common enough that most people will know at least something about what youre discussing. Technology, for example, is a good topic because its something we can all relate to in one way or another. Once youve chosen a topic, you must narrow it down into a single  thesis or central idea. The thesis is the position youre taking in relation to your topic or a related issue. It should be specific enough that you can bolster it with just a few relevant facts and supporting statements. Think about an issue that most people can relate to, such as:  Technology is changing our lives. Drafting the Outline Once youve selected your topic and thesis, its time to create a roadmap for your essay that will guide you from the introduction to conclusion. This map, called an outline, serves as a diagram for writing each paragraph of the essay, listing the three or four most important ideas that you want to convey. These ideas dont need to be written as complete sentences in the outline; thats what the actual essay is for. Heres one way of diagramming an essay on how technology is changing our lives: Introductory Paragraph Hook: Statistics on home workersThesis: Technology has changed workLinks to main ideas to be developed in the essay: Technology has changed where, how and when we work Body Paragraph I Main idea: Technology has changed where we can workSupport: Work on the road exampleSupport: Work from home example statisticConclusion Body Paragraph II Main idea: Technology has changed how we workSupport: Technology allows us to do more on our own example of multitaskingSupport: Technology allows us to test our ideas in simulation example of digital weather forecastingConclusion Body Paragraph III Main idea: Technology has changed when we workSupport: Flexible work schedules example of telecommuters working 24/7Support: Technology allows us to work any time example of people teaching online from homeConclusion Concluding Paragraph Review of main ideas of each paragraphRestatement of thesis: Technology has changed how we workConcluding thought: Technology will continue to change us Note that the author uses only three or four main ideas per paragraph, each with a main idea, supporting statements, and a summary.   Creating the Introduction Once youve written and refined your outline, its time to write the essay. Begin with the  introductory paragraph. This is your opportunity to hook the readers interest in the very first sentence, which can be an interesting fact, a quotation, or a  rhetorical question, for instance. After this first sentence, add your thesis statement. The thesis clearly states what you hope to express in the essay. Follow that with a sentence to introduce your  body paragraphs. This not only gives the essay structure, but it also signals to the reader what is to come. For example: Forbes magazine reports that One in five Americans work from home. Does that number surprise you? Information technology has revolutionized the way we work. Not only can we work almost anywhere, we can also work at any hour of the day. Also, the way we work has changed greatly through the introduction of information technology into the workplace. Notice how the author uses a fact and addresses the reader directly to grab their attention. Writing the Body of the Essay Once youve written the introduction, its time to develop the meat of your thesis in three or four paragraphs. Each should contain a single main idea, following the outline you prepared earlier. Use two or three sentences to support the main idea, citing specific examples. Conclude each paragraph with a sentence that summarizes the argument youve made in the paragraph.   Lets consider how the location of where we work has changed. In the past, workers were required to commute to work. These days, many can choose to work from the home. From Portland, Ore., to Portland, Maine, you will find employees working for companies located hundreds or even thousands of miles away. Too, the use of robotics to manufacture products has led to employees spending more time behind a computer screen than on the production line. Whether its in the countryside or in the city, youll find people working everywhere they can get online. No wonder we see so many people working at cafes! In this case, the author continues to directly address the reader while offering examples to support their assertion. Concluding the Essay The summary paragraph summarizes your essay and is often a reverse of the introductory paragraph.  Begin the summary paragraph by quickly restating the principal ideas of your body paragraphs. The penultimate (next to last)  sentence should restate your basic thesis of the essay. Your final statement can be a future prediction based on what you have shown in the essay.   In this example, the author concludes by making a prediction based on the arguments made in the essay. Information technology has changed the time, place and manner in which we work. In short, information technology has made the computer into our office. As we continue to use new technologies, we will continue to see change. However, our need to work in order to lead happy and productive lives will never change. The where, when and how we work will never change the reason why we work.